Cytokine mRNA expression in C/EBPε−/− macrophages.

Macrophages were harvested from PL 24 hours after thioglycollate challenge and from bone marrow cells cultured for 13 days with and without stimulation with 2-hour LPS (1 μg/mL) plus IFN-γ (100 U/mL) stimulation. Macrophage-mediated cytokines were measured by RT-PCR because of the low basal level of expression. PCR products were blotted and hybridized using internal oligonucleotide ³²P-labeled probes specific for the amplified genes.