Fig. 3.
Effect of thrombin on Ang-2 protein production and secretion.
FS4 and HUVE cells were cultured in media with or without thrombin, 1 U/mL, for 8 hours at 37°C. The media were collected and concentrated and the cells extracted with lysis buffer. Both were then analyzed for Ang-2 production by Western blot. (A) Ang-2 in HUVE cells, not in FS4 cells. (B) Ang-2 in supernatant of HUVE cells.
