Effect of signaling pathway protein kinase inhibitors on thrombin-induced expression of Ang-2 mRNA.

Cells were treated without (C) or with wortmannin (W) 1 μM, H7 (20 μM), or PD98059 (100 μM) for 2.5 hours at 37°C and thrombin, 1 U/mL, added for an additional 3 hours. Northern blot was then performed for Ang-2 mRNA. Note inhibition by H7 and PD98059, which inhibit serine/threonine kinases and the MAPK pathway.