Fig. 7.
Effect of thrombin-induced Ang-2 on binding of Ang-1 to its Tie-2 receptor.
HUVE cells were treated with 1 U/mL thrombin for 5 hours and the concentrated supernatant incubated with 35S–Ang-1 prior to binding of Ang-1 to Tie-2–Fc. Binding was analyzed by autoradiography following immunoprecipitation with protein A and 12% SDS–polyacrylamide gel electrophoresis. Lane 1, 35S–Ang-1 plus Tie-2–Fc. Lane 2, 35S–Ang-1 plus supernatant of thrombin-hirudin–treated HUVE cells. Lane 3, 35S–Ang-1 plus supernatant of thrombin-treated HUVE cells. Lane 4, absence of Tie-2–Fc in the system. Representative of 3 experiments.
