Induction of anti-ALK–specific effectors in healthy donor PBLs.

PBLs were activated with autologous peptide-pulsed DCs and restimulated weekly with peptide-loaded autologous monocytes. The resulting T-cell bulk cultures were tested for antigen specificity starting at the third round of stimulation. Cytotoxic activity was determined in a standard⁵¹Cr release assay at an E/T ratio of 50:1 (▪) and 25:1 (■) using as targets T2 cells pulsed with 5 μM cognate peptide or irrelevant influenza matrix-derived peptide (FLU) (A) and NPM/ALK⁺ lymphoma cell lines SU-DHL1 and SUP-M2 (B). Results of 1 representative experiment of 2 performed are shown.