U-937 cells arrest in the G0/G1phase of the cell cycle and up-regulate the differentiation marker CD11c in response to ATRA treatment.
(A) The graph indicates the percentage of cells in the S, G2, and M phases during ATRA differentiation. U-937 cells were grown in the presence of ATRA and collected at the indicated times, and the distribution of cells in different cell cycle phases was determined by flow cytometry of PI-stained nuclei. Mean ± SD (n = 4). (B) Induction of CD11c expression during ATRA-induced differentiation. Cells were induced by ATRA, collected at the indicated times, and analyzed by flow cytometry. Data are presented as CD11c mean fluorescence intensity (MFI) corrected for background binding by subtracting the MFI value for isotype-specific IgG2b control antibody. Mean ± SD (n = 3).
