American Society of Hematology

Fig. 4.

$Fig. 4. Ca++-dependent association of synexin and sorcin with microvesicular lipid rafts. / Microvesicles prepared according to method B (10-minute low-speed centrifugation) were lysed with cold 1% Triton X-100 in TBS, in the presence of Ca++ or EDTA, and subjected to discontinuous density gradient centrifugation. Thirteen fractions were collected from the top, and aliquots were analyzed by 12% polyacrylamide gel electrophoresis/silver staining (panel A) and immunoblotting (panel B), as indicated, and for AChE activity and cholesterol content (percentage of total).$

Ca⁺⁺-dependent association of synexin and sorcin with microvesicular lipid rafts.

Microvesicles prepared according to method B (10-minute low-speed centrifugation) were lysed with cold 1% Triton X-100 in TBS, in the presence of Ca⁺⁺ or EDTA, and subjected to discontinuous density gradient centrifugation. Thirteen fractions were collected from the top, and aliquots were analyzed by 12% polyacrylamide gel electrophoresis/silver staining (panel A) and immunoblotting (panel B), as indicated, and for AChE activity and cholesterol content (percentage of total).

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