American Society of Hematology

Fig. 1.

$Fig. 1. Evaluation of antiheparin antibody specificity by ELISA. / Periplasmic fractions containing antiheparin antibodies were applied to various GAG species coated on microtiter plates. Bound antibodies were detected using anti-VSV mouse monoclonal antibody P5D4, followed by alkaline phosphatase–conjugated rabbit antimouse IgG. Enzymatic activity was measured using p-nitrophenyl phosphate as a substrate. Bars represent the mean reactivity ± SD (n = 3) of the antibodies in percent relative to their reactivity with heparin. (A) Reactivity toward HS from bovine kidney. (B) Reactivity toward HS from porcine intestinal mucosa. (C) Reactivity toward chondroitin-6 sulfate from shark cartilage.$

Evaluation of antiheparin antibody specificity by ELISA.

Periplasmic fractions containing antiheparin antibodies were applied to various GAG species coated on microtiter plates. Bound antibodies were detected using anti-VSV mouse monoclonal antibody P5D4, followed by alkaline phosphatase–conjugated rabbit antimouse IgG. Enzymatic activity was measured using p-nitrophenyl phosphate as a substrate. Bars represent the mean reactivity ± SD (n = 3) of the antibodies in percent relative to their reactivity with heparin. (A) Reactivity toward HS from bovine kidney. (B) Reactivity toward HS from porcine intestinal mucosa. (C) Reactivity toward chondroitin-6 sulfate from shark cartilage.

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