American Society of Hematology

Fig. 6.

$Fig. 6. Confocal microscopical analysis of lung stained with antiheparin antibodies. / Cryosections of human lung were incubated with periplasmic fractions of antiheparin antibodies EW4E1 (A) and EW4G1 (C). Bound antibodies were visualized by incubation with rabbit polyclonal anti–c-Myc IgG followed by Alexa 488–conjugated goat antirabbit IgG. Tryptase, present in mast cells, was visualized using mouse antihuman mast cell tryptase followed by Alexa 594–conjugated goat antimouse IgG (B, D). Both antiheparin antibodies react strongly with mast cells. Scale bar, 100 μm.$

Confocal microscopical analysis of lung stained with antiheparin antibodies.

Cryosections of human lung were incubated with periplasmic fractions of antiheparin antibodies EW4E1 (A) and EW4G1 (C). Bound antibodies were visualized by incubation with rabbit polyclonal anti–c-Myc IgG followed by Alexa 488–conjugated goat antirabbit IgG. Tryptase, present in mast cells, was visualized using mouse antihuman mast cell tryptase followed by Alexa 594–conjugated goat antimouse IgG (B, D). Both antiheparin antibodies react strongly with mast cells. Scale bar, 100 μm.

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