Northern blotting experiments confirm the quantitative decrease in GATA-2 transcript levels.

RNA was isolated from normal (N) or VAD (V) embryos at stage 9 or 10, and mRNA was analyzed by Northern blotting using probes to GATA-1, GATA-2, or control EF-1β cDNA as indicated. Note that there are 2 GATA-2 transcripts (arrowheads), and levels of both are decreased significantly at these stages relative to either GATA-1 or EF-1β.