Fig. 3.
Fig. 3. Time studies on the cleavage of α1m. / A quantity of 0.5 μg plasma-α1m was incubated with lysed erythrocytes, erythrocyte membranes, or PBS (control) at 37°C for indicated time points: 1, 5, 30, 60, and 240 minutes. The cleavage reaction was stopped by adding SDS-PAGE sample buffer and was analyzed by SDS-PAGE in the presence of mercaptoethanol and Western blotting with anti-α1m and anti-LIPR.

Time studies on the cleavage of α1m.

A quantity of 0.5 μg plasma-α1m was incubated with lysed erythrocytes, erythrocyte membranes, or PBS (control) at 37°C for indicated time points: 1, 5, 30, 60, and 240 minutes. The cleavage reaction was stopped by adding SDS-PAGE sample buffer and was analyzed by SDS-PAGE in the presence of mercaptoethanol and Western blotting with anti-α1m and anti-LIPR.

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