Fig. 6.
Fig. 6. SDS-PAGE of urinary α1m. / Urinary α1m was purified by affinity chromatography on a column with monoclonal antibodies against α1m. The eluate was analyzed by SDS-PAGE in the presence of mercaptoethanol (T = 12%, C = 3,3), staining, and Western blotting with anti-α1m and anti LIPR. Urine from apparently healthy donors (lanes 4-6) as well as patients having different disorders associated with the following changes in erythrocyte formation and destruction were used: lane 1, suspected mechanical hemolytic anemia associated with mitral valve reconstruction following endocarditis; lane 2, autoimmune hemolytic anemia (warm type, IgG-mediated); lane 3, paroxysmal nocturnal hemoglobinuria and myelodysplastic syndrome.

SDS-PAGE of urinary α1m.

Urinary α1m was purified by affinity chromatography on a column with monoclonal antibodies against α1m. The eluate was analyzed by SDS-PAGE in the presence of mercaptoethanol (T = 12%, C = 3,3), staining, and Western blotting with anti-α1m and anti LIPR. Urine from apparently healthy donors (lanes 4-6) as well as patients having different disorders associated with the following changes in erythrocyte formation and destruction were used: lane 1, suspected mechanical hemolytic anemia associated with mitral valve reconstruction following endocarditis; lane 2, autoimmune hemolytic anemia (warm type, IgG-mediated); lane 3, paroxysmal nocturnal hemoglobinuria and myelodysplastic syndrome.

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