Fig. 7.
![Fig. 7. Time-dependence of [14C]heme-binding to α1m. / Approximately 0.2 nmol plasma-α1m, t-α1m, human serum albumin (HSA), and orosomucoid was incubated with 25 pmol [14C]heme for 1, 3, 15, 60, and 180 minutes. The binding of [14C]heme to the proteins was then determined by SDS-PAGE, staining (A), and phosphoimaging (B).](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/99/6/10.1182_blood.v99.6.1894/6/h80622232007.jpeg?Expires=1735512517&Signature=sQlTmskhNkr5v-oThe8EXnqmUHwWEO11pGCf3vIChBWpgVcyt2UrpA6G8jV8BSH-FEGu-WfYARHEBdzDK6RSKktCQvpi8VDsMDpShrCSja~PHUT6RvUNo9CSytHopJf-zHGuG31q86bK-Mhvp5bWIWqyYhXtZaJOByauJuawCKp4JNkbceGQfSomiJCKOFFioP11EuuRRIj992SqwBk-tDAP5zc2MqyN57oHQ6JpdiwRyW9C3c3-mcrecfBQkamJz1-Id7IRpQA4yXlpJ-rH6giWGQ7TR5YZPYGzoWM~G7i903~Ru3pb3~oGYvFXMpHpWlZTEytqk4ftB2Qp26VcVQ__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Time-dependence of [14C]heme-binding to α1m.
Approximately 0.2 nmol plasma-α1m, t-α1m, human serum albumin (HSA), and orosomucoid was incubated with 25 pmol [14C]heme for 1, 3, 15, 60, and 180 minutes. The binding of [14C]heme to the proteins was then determined by SDS-PAGE, staining (A), and phosphoimaging (B).
