VEGF increases Bcl-2, but not Bax, expression by interacting with KDR, but not FLT-1.

(A) Protein extracts were obtained from HL-60 cells, which were cultured in serum-free RPMI for 24 hours (control), in presence of 50 ng/mL VEGF, VEGF plus 1 μg/mL KDR antibody (clone IMC-1C11), VEGF plus 1 μg/mL FLT-1 antibody (clone 6.12), or 100 ng/mL (PLGF). (B) Western blotting results were quantified by densitometry and are shown as the ratio between densitometry values in treated versus untreated (control) cells. Each experiment was repeated 3 times, and the SD between the different ratios was calculated and is shown.