GA blocks VEGF-induced Bcl-2 expression on HL-60 cells.

HL-60 cells were cultured in serum-free RPMI for 24 hours (control), in the presence of 50 ng/mL VEGF₁₆₅ (VEGF) and combination of VEGF₁₆₅ and 0.02 μM geldanamycin (VEGF + GA). Western blotting results were quantified by densitometry and are shown as the ratio between densitometry values in treated versus untreated (control) cells. Each experiment was repeated 3 times, and the SD between the different ratios was calculated and is shown. Double asterisks indicate that difference between VEGF plus GA and VEGF alone is significant (P < .05).