American Society of Hematology

Fig. 1.

$Fig. 1. CA-4-P disrupts the endothelial microtubule cytoskeleton and alters endothelial cell morphology. / (A,B) Immunostaining of endothelial cells with an anti–β-tubulin antibody before (A) and after (B) exposure to CA-4-P (1 μM, 30 minutes). Bar = 60 μm. (C,D) Western blotting analysis of soluble (S) and polymeric (P) tubulin fractions. Cells were exposed to either CA-4-P (30 minutes) or Taxol (30 minutes; C) or t-CA-4-P (30 minutes) or indicated amounts of CA-4-P (30 minutes; D). Equal aliquots of protein (30 μL) were run into the gel. (E,F) Phase contrast images of endothelial cells before (E) or after (F) exposure to CA-4-P (1 μM, 30 minutes). Arrowheads indicate blebbing cells. Bar = 90 μm.$

CA-4-P disrupts the endothelial microtubule cytoskeleton and alters endothelial cell morphology.

(A,B) Immunostaining of endothelial cells with an anti–β-tubulin antibody before (A) and after (B) exposure to CA-4-P (1 μM, 30 minutes). Bar = 60 μm. (C,D) Western blotting analysis of soluble (S) and polymeric (P) tubulin fractions. Cells were exposed to either CA-4-P (30 minutes) or Taxol (30 minutes; C) or t-CA-4-P (30 minutes) or indicated amounts of CA-4-P (30 minutes; D). Equal aliquots of protein (30 μL) were run into the gel. (E,F) Phase contrast images of endothelial cells before (E) or after (F) exposure to CA-4-P (1 μM, 30 minutes). Arrowheads indicate blebbing cells. Bar = 90 μm.

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