CA-4-P stimulates actin stress fiber formation via Rho/Rho-kinase.

Sparsely plated endothelial cells were pretreated with either vehicle control (30 minutes; A,B) or C3 exoenzyme (24 hours, 10 μg/mL; C,D) or Y-27632 (30 minutes, 10 μM; E,F) or HA1077 (30 minutes, 10 μM; G,H) and then were exposed to 1 μM CA-4-P for 30 minutes (B,D,F,H). Actin was stained with Texas Red–conjugated phalloidin as described in “Materials and methods,” and cells were examined by fluorescence microscopy. Bar = 30 μm.