CA-4-P induces the assembly of focal adhesions.

Sparse endothelial cells grown on Permanox slides were exposed to CA-4-P (60 minutes, 1 μM). Cells in panels E and F were pretreated with PD98059 (30 minutes, 25 μM) to induce blebbing. Cells were fixed and double stained with Texas Red phalloidin and antivinculin antibody. Panels A,C,E have vinculin staining; panels B,D,F, actin staining. Bar = 20 μm.