Fas/Fas-L pathway is not involved in the lysis of UT-7 and UT-7/9 cells.

(A) Fas expression on UT-7 (left panel) and UT-7/9 (right panel) cell lines (ZB4 mAb). (B) Calcium-dependent and calcium-independent lysis of UT-7/9 transfectants by dNK cells. Cytotoxic activity of dNK cells toward UT-7 and UT-7/9 cells was measured either in media or in the presence of MgCl₂ (3 mM) and EGTA (4 mM) at the indicated E/T ratio. One representative experiment of 3 is shown. (C) Proliferation assessed by MTT assay of UT-7 and UT-7/9 cells after a 24-hour incubation with anti-Fas mAb (CH-11 indicated by black bars) or with control IgM (gray bars).