UT-7/9 cells display a constitutive activation of NF-κB that is modulated by STI571.

(A) EMSA showing NF-κB activation in UT-7, UT-7/G6, and UT-7/9 cells. (B) NF-κB activity determined by NF-κB–dependent reporter gene. Cells were transfected with a plasmid containing a NF-κB LUC reporter (3 × NF-κB(pIC)-tk-LUC) using the Fugene system and luciferase activity measured 30 hours after transfection. (C) Western blot analysis of IκB phosphorylation in the 3 cell lines.