Fig. 1.
Fig. 1. Profile migration of osmotic lysis curves of oxy and deoxy AA and SS discocytes: effects of vanadate and [Ca2+]oon RBC volume distributions. / RBCs were suspended at 10% Hct in solution C containing 2 mM [Ca2+]o and incubated at 37°C in a tonometer. At time = 0, vanadate (1 mM) was added to all suspensions except the controls. Samples for hemolysis curves were taken at the indicated times after vanadate addition. Percent lysis is plotted as a function of RT (see “Materials and methods”). The RBC type and suspension conditions are indicated in the figure.

Profile migration of osmotic lysis curves of oxy and deoxy AA and SS discocytes: effects of vanadate and [Ca2+]oon RBC volume distributions.

RBCs were suspended at 10% Hct in solution C containing 2 mM [Ca2+]o and incubated at 37°C in a tonometer. At time = 0, vanadate (1 mM) was added to all suspensions except the controls. Samples for hemolysis curves were taken at the indicated times after vanadate addition. Percent lysis is plotted as a function of RT (see “Materials and methods”). The RBC type and suspension conditions are indicated in the figure.

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