Fig. 4.
![Fig. 4. Profile migration of osmotic lysis curves of SS discocytes. / Comparison of dehydration patterns induced by permeabilization to K+ with valinomycin (A), A23187 plus Ca2+ (B), and deoxy (D) versus oxy incubation (C). Cells were suspended at 10% Hct in solution C with 1 mM [Ca2+]o, incubated at 37°C in a tonometer, and at time = 0, 1 mM vanadate was added in the oxy-deoxy conditions (C,D). The cells used for the experiments of panels C and D were from the same donor; those in panels A and B were from different donors. The differences in the control curves of panels A-C reflect the interdonor variation regularly observed in hemolysis curves with sickle discocyte fractions.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/99/7/10.1182_blood.v99.7.2578/6/h80722340004.jpeg?Expires=1735836492&Signature=JuznWLJAL2Ubbrfet6SawJ3Gy0NwnclsWQFwCC26fpRVsclekx26BHdHw9eXrUJ7pDwn~i-T9fxPdsvIWZjNeQIqUGDGZtBpvuzfF1L9tplgGpeLLuWW~kzjtWVdJRClX3-QbXLjlnHs2PdSLyTkLXY8hiZpOtPjxEY6OkBwlUAoAT~vbYAIj0M3k7ZvOe~nrA4zX4mF43DyLMPVHy6aiJnrG6oaPAWNX1NDLtieMbcAKCKTwdId-Jpk8mQgPE~DmhCzJG0nEvhfXBIkrUVJpW~AzuRJZmK2TvGcyaCboqhFDFkDgMj6CSBMUwH35w6CqQKEaVhOuqHSa8omK9lkDQ__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Profile migration of osmotic lysis curves of SS discocytes.
Comparison of dehydration patterns induced by permeabilization to K+ with valinomycin (A), A23187 plus Ca2+ (B), and deoxy (D) versus oxy incubation (C). Cells were suspended at 10% Hct in solution C with 1 mM [Ca2+]o, incubated at 37°C in a tonometer, and at time = 0, 1 mM vanadate was added in the oxy-deoxy conditions (C,D). The cells used for the experiments of panels C and D were from the same donor; those in panels A and B were from different donors. The differences in the control curves of panels A-C reflect the interdonor variation regularly observed in hemolysis curves with sickle discocyte fractions.
