The effect of cytochalasin D on the apparent stiffness of quiescent neutrophils and neutrophils stimulated with fMLP.

Pretreatment with cytochalasin D (10 μM) decreased the apparent stiffness of quiescent neutrophils (time 0). This decrease was greater for neutrophils isolated from the BM than the circulating blood, and there was no significant difference in the stiffness of neutrophils isolated from the BM or the circulating blood after this pretreatment. In addition, cytochalasin D completely inhibited the increase in stiffness induced by fMLP (P < .05). n = 5 rats in each group. BM indicates bone marrow; C, circulating.