American Society of Hematology

Fig. 1.

$Fig. 1. Isolation and characterization of CD34 and its glycans. / (A) Western blot shows that human tonsillar endothelial CD34 reacts with MECA-79. Lanes 1 and 4 are after the WGA column, lanes 2 and 5 after the CD34 mAB 581 column, and lanes 3 and 6 after desalting and concentration. The arrow indicates the CD34 at 105 kd. (B) Proposal for structures of human tosillar high endothelial CD34 O-glycans of the sLex containing fraction O-4 and sulfated sLex containing fraction O-9. The latter putatively bears both 6-sulfo-sLex and MECA-79 epitopes.$

Isolation and characterization of CD34 and its glycans.

(A) Western blot shows that human tonsillar endothelial CD34 reacts with MECA-79. Lanes 1 and 4 are after the WGA column, lanes 2 and 5 after the CD34 mAB 581 column, and lanes 3 and 6 after desalting and concentration. The arrow indicates the CD34 at 105 kd. (B) Proposal for structures of human tosillar high endothelial CD34 O-glycans of the sLex containing fraction O-4 and sulfated sLex containing fraction O-9. The latter putatively bears both 6-sulfo-sLex and MECA-79 epitopes.

This Feature Is Available To Subscribers Only