Adhesion and migration of cultured CD34⁺residing in different phases of the cell cycle.

CB CD34⁺ cells were cultured ex vivo for 48 hours and assayed for Fn binding (n = 18 [A]) or migration toward MS-5 CM across Fn- (n = 6 [B]) or BSA-coated filters (n = 6 [C]). Percent adhesion or migration of cells in G₀/G₁, S, and G₂/M was calculated relative to the total number of cells in each phase in the input population. (A) *Adhesion was statistically higher in S than in G₀/G₁ (P < .05); **adhesion was statistically higher in G₂/M than in G₀/G₁ and in S (P < .05). (B) *Migration was statistically lower in S than in G₀/G₁ (P < .05); **migration was statistically lower in G₂/M than in G₀/G₁ and in S (P < .05)