American Society of Hematology

Fig. 6.

$Fig. 6. Adhesion and migration of FACS-sorted cycling and noncycling CD34+ cells. / Cultured CD34+ cells were fractionated into noncycling (G0/G1) and cycling (S/G2/M) fractions by Hoechst sorting (n = 3). (A) Transmigration of cycling and noncycling cells toward MS-5 CM was determined across Fn- or BSA-coated filters. *Migration of noncycling cells was higher than that of cycling cells (P < .0.5); **migration of cycling cells across Fn was lower than across BSA (P < .0.5). (B) Both fractions were assayed for adhesion to Fn- or BSA-coated plates. *Fn binding of cycling cells was higher than that of noncycling cells (P < .05). ND indicates not detected.$

Adhesion and migration of FACS-sorted cycling and noncycling CD34⁺ cells.

Cultured CD34⁺ cells were fractionated into noncycling (G₀/G₁) and cycling (S/G₂/M) fractions by Hoechst sorting (n = 3). (A) Transmigration of cycling and noncycling cells toward MS-5 CM was determined across Fn- or BSA-coated filters. *Migration of noncycling cells was higher than that of cycling cells (P < .0.5); **migration of cycling cells across Fn was lower than across BSA (P < .0.5). (B) Both fractions were assayed for adhesion to Fn- or BSA-coated plates. *Fn binding of cycling cells was higher than that of noncycling cells (P < .05). ND indicates not detected.

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