Fractionation of cultured CD34⁺ cells on the basis of VLA-5 expression and cell cycle status.

Cultured CD34⁺ cells were simultaneously labeled with anti–VLA-5 and Hoechst 33342. (A) VLA-5 histogram showing gating of CD34⁺ VLA^low (R1) and VLA-5^high(R2) cells. (B) DNA histogram of total CD34⁺ cells. (C) DNA histogram of CD34⁺VLA-5^low cells. (D) DNA histogram of CD34⁺VLA-5^high cells. In each of VLA-5^low and VLA-5^high subsets, noncycling (R3) and cycling (R4) CD34⁺ cells were sorted.