Fig. 3.
Platelet prothrombinase activity in response to agonist treatment.
Platelet suspensions from control dogs or affected GSDs were either treated with dimethyl sulfoxide (UnStm) or stimulated with 0.5 U/mL thrombin (Thr), 0.5 U/mL thrombin combined with 24 μg/mL collagen (Thr/Coll), or 3 μM calcium ionophore (A23187). Platelets were stirred with the various compounds for 25 minutes and then tested in the prothrombinase assay as described in “Materials and methods.” The nanomoles of thrombin generated by 108 platelets was determined for a 10-minute prothrombinase assay reaction time. Data represent mean values (with SD) obtained from testing 5 control dogs and 5 affected GSDs.
