Fig. 2.
Fig. 2. Shear-dependent morphology of VWF adsorbed onto collagen. / PBS containing 1 μg/mL VWF was perfused at 0.07 N/m2(A-C) or at 4.55 N/m2 (D-F) shear stress for 5 minutes over calf skin collagen-coated coverslips. No morphologic differences are apparent between the samples obtained at low or at high shear regimes. Double labeling with 15-nm and 30-nm gold-conjugated protein A (the black arrow points at a 15-nm gold bead, the white arrow shows a 30-nm gold particle). Tapping-mode AFM, Microlever “F” tip, F = 118 kHz, setpoint is approximately 90% of the free vibration amplitude. Image dimensions: 5 × 5-μm lateral and 40-nm height range.

Shear-dependent morphology of VWF adsorbed onto collagen.

PBS containing 1 μg/mL VWF was perfused at 0.07 N/m2(A-C) or at 4.55 N/m2 (D-F) shear stress for 5 minutes over calf skin collagen-coated coverslips. No morphologic differences are apparent between the samples obtained at low or at high shear regimes. Double labeling with 15-nm and 30-nm gold-conjugated protein A (the black arrow points at a 15-nm gold bead, the white arrow shows a 30-nm gold particle). Tapping-mode AFM, Microlever “F” tip, F = 118 kHz, setpoint is approximately 90% of the free vibration amplitude. Image dimensions: 5 × 5-μm lateral and 40-nm height range.

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