Sustained amelioration of hematologic parameters in bone marrow chimeras reconstituted with TNS9-transduced Hbb^th3/+bone marrow cells.

(A) Hemoglobin levels, hematocrit, RBC counts, and reticulocyte counts are shown at weeks 15, 30, and 40 after transplantation. All measured parameters show significant increases in recipients of TNS9-transduced Hbb^th3/+ versus eGFP-transduced Hbb^th3/+ bone marrow cells at week 40 after transplantation (P = .03 for each parameter). (B) Colony-forming assays were performed using spleen cells isolated from age-matched Hbb^+/+ (black fill) and Hbb^th3/+ mice (horizontal lines), along with cells from chimeras engrafted with eGFP-transduced Hbb^+/+ (white fill), eGFP-transduced Hbb^th3/+ (vertical lines), or TNS9-transuced Hbb^th3/+ (gray fill) bone marrow cells. CFU-E, BFU-E, and CFU-GM colonies were analyzed 40 weeks after transplantation (TNS9-transduced Hbb^th3/+ versus eGFP-transduced Hbb^th3/+ CFU-E, BFU-E, and CFU-GM,P = .03).