Fig. 1.
Characterization of mIL-17–transfected P815 and J558L cell lines.
(A) The cDNA derived from mRNA extracted from wild-type, mock-transfected (NT-Neo), or mIL-17–transfected tumor cells (P815 or J558L) was amplified by PCR using primers specific for HGPRT or IL-17 mRNA. Amplified PCR products were then loaded on a 2% agarose gel and stained with ethidium bromide for UV visualization. (B) Wild-type, mock-transfected, or IL-17–transfected tumor cells (P815 or J558L) were plated on 24-well flat-bottomed plates at a density of 5 × 105 cells per well. The cells were cultured for 72 hours, and IL-17 concentrations in the supernatants were measured by ELISA.
