Fig. 4.
In vivo persistence of the IL-17 transgene in tumor transfected with mIL-17 cDNA and transplanted into mice.
The cDNA derived from mRNA extracted from mock-transfected (NT-Neo) or IL-17–transfected P815 or J558L cells was amplified by PCR using primers specific for murine HGPRT (A) or mIL-17 (B). Amplified PCR products were loaded on a 2% agarose gel and stained with ethidium bromide for UV visualization.