IL-17 increases the generation of P815-specific CTLs.
DBA/2 mice were given a subcutaneous injection of live P815–IL-17 or P815 Neo cells at 2 sites (5 × 105 cells/site). Fifteen days later, whole PB was collected and stimulated in vitro with irradiated P815. CTL activity was measured 7 days later by using a51Cr-release assay on targets P815 or P815 A-E−, an antigen-loss variant of P815 that had lost the 5 immunodominant antigens A, B, C, D, and E. Each point represents the lytic activity of the PB lymphocytes from an individual mouse. To measure induction of CTLs against the immunodominant antigens specifically, we used a 50-fold excess of cold P815 A-E− cells during the assay. Data are expressed as P815 ABCDE–specific LUs/106 effector cells, obtained after subtraction of the LUs measured against the targets negative for P815 A-E−.
