Fig. 1.
![Fig. 1. Phenotype and function of FC/4TOO fusion cells. / (A) DCs, 4TOO plasmacytoma, and FC/4TOO fusion cells were analyzed by bidimensional flow cytometry for PKH-26 fluorescence and expression of the indicated antigens. (B) DCs, 4TOO cells, and FC/4TOO fusion cells were analyzed by bidimensional flow cytometry for the expression of MHC class II and Syndecan. (C) DCs (●), 4TOO plasmacytoma (○), FC/4TOO fusion cells (▴), and medium alone (□) were cocultured with C57BL/6 T cells at different ratios for 5 days. [3H]-thymidine uptake was measured at 12 hours after a pulse of 0.037 MBq (1 μCi)/well. The stimulation index is expressed as the mean ± SE of 3 experiments each performed in triplicate.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/99/7/10.1182_blood.v99.7.2512/6/h80722348001.jpeg?Expires=1735500062&Signature=v4dw3rtPwi7wGjdRvY9e2osFXHdvlzsVh0pf4COVKI482UmkldtqXZ5bOGpOFZ461hjsylN8gbKnyA9kWe3hzuVOm8QvAtknVFUuP0c2biK0DwL0g8A3qoJvqwuriUTZW1jT8jNL11AKcEDaTxdhZYg~eD9IN4iJAQJwYn8BtTrhabdmlYr4g6Gf25d4-uJ60HuOr1PbYpvryps-r2RnyEZ~YSoDzNcCtN1~xz3rvBjAq2yLzLm4K0fSB-WZJ49CZEQHiBS-4uRShmnBqS8MQ4xUyaToClCkVCZBapJXfh8w4s5d~UuRRplOK0~NdnB26h6DczezEt5ZtxeKSy-r9w__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Phenotype and function of FC/4TOO fusion cells.
(A) DCs, 4TOO plasmacytoma, and FC/4TOO fusion cells were analyzed by bidimensional flow cytometry for PKH-26 fluorescence and expression of the indicated antigens. (B) DCs, 4TOO cells, and FC/4TOO fusion cells were analyzed by bidimensional flow cytometry for the expression of MHC class II and Syndecan. (C) DCs (●), 4TOO plasmacytoma (○), FC/4TOO fusion cells (▴), and medium alone (□) were cocultured with C57BL/6 T cells at different ratios for 5 days. [3H]-thymidine uptake was measured at 12 hours after a pulse of 0.037 MBq (1 μCi)/well. The stimulation index is expressed as the mean ± SE of 3 experiments each performed in triplicate.
