Fig. 1.
RT-PCR analysis of eosinophil neurotrophin gene expression.
(A) Total mRNA was extracted from 2 × 106 freshly isolated eosinophils or cultured HMC-1 cells. The RNA was reverse transcribed to complementary DNA and amplified with specific primers for NGF, NT-3, or β2-MG. A 35-cycle PCR was performed. The products were electrophoresed on a 3% agarose gel followed by staining with ethidium bromide. (B) The experiments were repeated 3 times using eosinophil preparations from 3 different donors. Amplified complementary DNA bands are compatible with the size of estimated PCR products for NGF (437 bp), NT-3 (459 bp), and β2-MG (positive control, 320 bp).
