Effects of cellular stimulation with immune complexes and/or IL-5 on secretion of NGF by eosinophils.
Eosinophils (1 × 106 cells) were suspended in 1 mL RPMI 1640 medium supplemented with 10% DCS and incubated with medium alone or 20 μg/mL sIgA, serum IgA, or serum IgG for 1 hour at 4°C. Cells were then stimulated with the corresponding antihuman sIgA monoclonal antibody, goat antihuman IgA, or goat antihuman IgG and cultured in the absence (open columns) or presence (closed columns) of 25 ng/mL IL-5 at 37°C for 24 hours. After culture, cell-free supernatants were collected, and the amounts of NGF were measured by ELISA. The data show mean ± SEM from 5 experiments. *denotes significant difference from the values cultured with medium alone (Med) (P < .05). †denotes significant difference from the values cultured with the same stimuli but without IL-5 (P < .05).
