Alignment of the promotor regions of human and rat PAI-1.

The promoter region of human PAI-1, stretching from position −806 to +19 relative to transcription start, was aligned with the homologous sequence from rat. The 5 putative HREs are highlighted in gray, and uppercase letters indicate homology with the canonical HRE sequence BACGTSSK, B = B = G/C/T, S = C/G, and K = G/T.23The rat HIF-1 and USF-2a binding sites are highlighted in black. Finally, the CACAG element that is important for the functionality of various HREs is boxed.29 In the rat sequence * denotes identical sequences, letters indicate substitutions, and deletions are represented by dashes.