Overexpression of wild-type, the PH domain, and a deletion mutant Gab2 without SHP-2 binding sites in Ba/F3 cells.

(A) Wild-type (Wt), the PH domain encoding region (PH), and Gab2 deletion mutant (Mt) cDNAs were cloned into MSCV-IR-GFP vector and transfected into Ba/F3 cells by electroporation. (B) GFP-expressing cells were sorted by FACS after 48 hours. Sorted cells were expanded and lysed in RIPA buffer, 50 μg cell lysates was resolved by SDS-PAGE and blotted with anti-Gab2 antibody.