Fig. 6.
BL cells express the serotonin transporter.
(A) Western blotting analysis for the expression of human SERT protein in HEK 293 cells transfected with human SERT, HEK 293 wild-type cells, and BL lines. Upper band indicates ectopically expressed human neuronal SERT (apparent molecular weight of 90 kd). Lower arrow indicates the approximate 70-kd hSERT immunoreactive protein in BL cells. The blot shown is representative of 3 different experiments. (B) 5-HT uptake was assayed for 0 to 60 minutes at 37°C, at a substrate concentration of 500 nM, in 2 × 105 L3055 cells. Data are the mean ± standard error of 4 experiments.
