Fig. 1.
Morphology of each population of live, apoptotic, and necrotic Jurkat cells after separation by cell sorting.
Jurkat cells (1 × 107) were treated with Camptothecin for 24 hours and were separated according to the binding of PE–annexin V and the uptake of 7-AAD into live, apoptotic, and necrotic populations by cell sorting using a FACSVantage machine and were stained with Giemsa-May-Grünwald. Typical morphology of each population was determined by light microscopy, as shown in the insets: (A) live cells with intact membrane and a rim of cytoplasm; (B) apoptotic cells with characteristic apoptotic bodies; (C) necrotic cells with swollen organelles and loss of a discrete cell membrane.
