Flow cytometric analysis of the binding of FITC-heparin to live, apoptotic, and necrotic cells derived from freshly prepared leukocytes.

Leukocytes were prepared from fresh blood and were treated with Camptothecin for 16 hours, as described in “Materials and methods.” Cells were labeled with FITC-heparin at a range of concentrations (0.5 nM-5000 nM) and then with PE–annexin V and 7-AAD. Live, apoptotic, and necrotic cells were discriminated by FACS analysis. Histogram plots of the binding to each population at 5 μM FITC-heparin are shown as relative fluorescence intensity on the x-axis.