Phenotype of DCs isolated from mice treated with G-CSF or pGM-CSF.

DCs isolated from spleens of C57BL/6 mice that were either untreated or treated for 10 days with G-CSF (10 μg/day) or for 5 days with pGM-CSF (2 μg/d), were immunofluorescent stained with antibodies directed to typical murine DC markers. The DCs were analyzed by gating on high forward light scatter and high levels of expression of CD11c. Data for each cytokine treatment are representative of 3 experiments that examined pooled splenic DCs from at least 3 mice.