Fig. 6.
IFN-γ–producing capacity of CD4−CD8− DCs from cytokine-treated mice.
Sorted subpopulations of CD4−CD8− splenic DCs (0.5 × 106/mL) from cytokine-treated mice were stimulated in culture with IL-12 and IL-18 (20 ng/mL each). Data for 2 and 5 days (d) of ProGP-1 treatment are shown. The FL data are from treatment with muFL, but treatment with huFL yielded similar results. Supernatants were harvested after 3 days and then analyzed by ELISA for IFN-γ production. Data are from 1 experiment; error bars represent the range of results with duplicate samples. Similar results were obtained in more than 5 experiments using DCs from mice treated with ProGP-1 and FL and in 3 experiments using DCs from mice treated with G-CSF and pGM-CSF.
