Stimulation capacity of DCs from cytokine-treated mice.
Purified DC populations (103 cells/well) from mice treated for 10 days with G-CSF, ProGP-1, or FL were tested for their ability to stimulate naive CD4+ T cells (20 × 103cells/well) in an allostimulatory MLR and compared with the same DC populations from untreated mice. For clarity, only the maximum value for thymidine uptake (day 3.5) is shown for untreated mice and only the muFL data are shown, although DCs from mice given huFL stimulated T cells to a similar extent. The average count per minute from triplicate values is shown for each time point; error bars represent the range of results with triplicate samples. Data are from 1 experiment that compared all DC populations from treated and untreated mice. Similar results were obtained in a second experiment that compared DCs from G-CSF–treated mice with DCs from untreated mice and in 3 additional experiments that compared DCs from ProGP-1–treated and FL-treated mice with controls.
