Fig. 5.
Fig. 5. Mitomycin C treatment partially rescues theflt-1−/− vascular phenotype. / Day 6 ES cell cultures were fixed (A,B), left untreated (C,D), or treated with mitomycin C (E-G). Some cultures (C-G) were differentiated for an additional 48 hours. Cultures were labeled with an antibody to PECAM (green), and some cultures (C-F) were also labeled with the mitotic marker antiphosphohistone H3 (red). Notice the abundance of phosphohistone H3–labeled figures in untreated (C-D) cultures compared with treated (E-F) cultures. (G) Example of a treatedflt-1−/− culture that morphologically resembled WT vasculature. (H) Quantitative image analysis of the PECAM+ area of day 8 WT (+/+) andflt-1−/− (−/−) cultures treated with mitomycin C (red) or left untreated (green). Each bar represents the average stained area from at least 3 wells stained with PECAM antibody. Magnification was ×10 except C (×20) and G (×4).

Mitomycin C treatment partially rescues theflt-1−/− vascular phenotype.

Day 6 ES cell cultures were fixed (A,B), left untreated (C,D), or treated with mitomycin C (E-G). Some cultures (C-G) were differentiated for an additional 48 hours. Cultures were labeled with an antibody to PECAM (green), and some cultures (C-F) were also labeled with the mitotic marker antiphosphohistone H3 (red). Notice the abundance of phosphohistone H3–labeled figures in untreated (C-D) cultures compared with treated (E-F) cultures. (G) Example of a treatedflt-1−/− culture that morphologically resembled WT vasculature. (H) Quantitative image analysis of the PECAM+ area of day 8 WT (+/+) andflt-1−/− (−/−) cultures treated with mitomycin C (red) or left untreated (green). Each bar represents the average stained area from at least 3 wells stained with PECAM antibody. Magnification was ×10 except C (×20) and G (×4).

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