Anatomic distribution of DC subsets.

Frozen spleen sections were processed for double labeling as described in “Materials and methods.” Blue (Fast Blue) = CD11c, and the indicated markers are shown in red (AEC). Double-labeled cells are purple: (A) Thy 1.2, (B) CD4 (arrows indicate CD11c⁺/CD4⁺ cells), (C) CD8 (arrows indicate CD11c⁺/CD8⁺ cells), and (D) CD4 and CD8 combination (arrows indicate CD11c⁺/CD4⁻/CD8⁻ cells). Asterisks indicate periarteriolar lymphoid sheath.