Fig. 7.
CTL-mediated killing of DC subsets.
Fresh C57BL/6 spleen DCs (2 × 105) were cultured with 5-day syngeneic (C57BL/6 CTL primed by irradiated Balb/c spleen cells) or allogeneic (Balb/c CTL primed by irradiated C57BL/6 spleen cells) CTL blasts at the indicated ratios. DC viability was determined 4 hours later for each of the CD8α− or CD8α+subsets as described in “Materials and methods” and is shown as a percent lysis of DCs cultured in the absence of CTLs.
