HUVECs produce VEGF-C.

(A) Primary HUVECs were cultured serum-free for 24 hours in the absence or presence of IL-1α (5 ng/mL) or FGF-2 (10 ng/mL). Supernatants were collected, immunoprecipitated for VEGF-C, and immunoblotted with antibodies against VEGF-C. Actin Western blot is also shown as a control for even protein concentration. (B) Densitometry measurements of total band intensities of the VEGF-C levels in HUVEC supernatants were measured and graphed. Error bars (SEM) were calculated from 3 independent experiments and represent the mean variation in signal intensity.