Induction of Bcl-2 levels by VEGF-C and mutVEGF-C.

(A) Serum-starved HEL and THP-1 cells were treated in the absence or presence of VEGF-C (100 ng/mL) or mutVEGF-C (200 ng/mL) for 24 hours. Cells were lysed in RIPA buffer, proteins resolved by acrylamide gel electrophoresis, and immunoblotted for Bcl-2 and Bax. (B) Bcl-2 and Bax band densities were measured, and Bcl-2/Bax ratios were graphed for the different conditions.