Responsiveness of human γδ T cells from bronchus–PLN cografts to in vivo EpoxPP challenge.

(A) Flow cytometry analysis using TCR-Cδ, -Vγ9, and -Vδ2–specific mAbs. Frequency (mean ± SD) of TCR-Cδ–positive cells is shown for mock-injected (open bar) and EpoxPP-injected (closed bar) grafts from two series. Among TCR-Cδ–positive cells from EpoxPP-injected grafts, most cells co-expressed TCR-Vγ9 (light grey bar) and TCR-Vδ2 (dark grey bar). (B) T cells derived from EpoxPP-injected (open circles) or noninjected (closed circles) bronchus–PLN cografts from the same series were incubated in vitro with EpoxPP (Ag) at various concentrations, and culture supernatants were tested for their tumor necrosis factor content. (C) Cytolytic activity of T cells derived from EpoxPP-injected (open circles) or noninjected (closed circles) bronchus–PLN cografts from the same series against ⁵¹Cr-labeled Vγ9Vδ/2-susceptible Daudi cells was estimated at 2 effector-to-target (E:T) ratios in a 4-hour⁵¹Cr-release assay.